Supporters of the Stroke Association will be walking 84 miles crossing Hadrian's Wall to build awareness about the illness and raise money to help sufferers, their families and carers.
The walk will last seven days, beginning on June 27th. The journey will begin at Wallsend and finish in Bowness.
Walkers will set the pace at between nine and 15 miles a day. Others will also have the chance to join just for one day of hiking.
Tessa Sanderson, Olympian and TV presenter, said: "Walking Hadrian's Wall is a fantastic challenge, not only to get fit and experience the stunning and historical landscape of the North but also to raise vital funds to beat the UK's third biggest killer.
Jon Barrick, chief executive of the Stroke Association, said: "Walking Hadrian's Wall will be tough but I know every step forward is money raised to help the 900,000 people living with the effects of stroke in the UK.
"As the leading cause of severe adult disability stroke can have a devastating impact on people's lives, I welcome walkers of all ages and abilities to join me and help us fight stroke together."